protein content  (Bio-Rad)

Journal: Food Science of Animal Resources

Article Title: Functional characteristics of peptides from whey proteins fermented with lactic acid bacteria isolated from Dongchimi

doi: 10.1007/s44463-025-00053-6

Figure Lengend Snippet: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) patterns of fermented WPC with DC10. Note: ( a ) Raw bovine milk. ( b ) Lane 1 contains Precision Plus Protein Dual Xtra standards (Bio-Rad, USA), which provide 12 pre-stained recombinant protein bands ranging from 2–250 kD, including nine blue-stained and three pink reference bands. Lanes 2, 3, 4, 5, and 6 represent hydrolysates obtained after 0, 4, 8, 12, and 16 h of hydrolysis, respectively. DC10 refers to Latilactobacillus sakei

Article Snippet: The operating conditions were set at 20 V for 20 min, followed by 100 V for 2 h. After staining with Coomassie blue R-250 (water: methanol: acetic acid [45:45:10, v/v/v]) and water: methanol: acetic acid (45:45:10, v/v/v) solutions, SDS-PAGE analysis was performed using Precision Plus Protein Dual Xtra Standards (Bio-Rad).

Techniques: Polyacrylamide Gel Electrophoresis, SDS Page, Staining, Recombinant